FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13535130
Sequences flagged as poor quality0
Sequence length105
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT368990.2726165171668096No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG255230.18856856195692248No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA229040.16921891404072217No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA191750.14166838441891583No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA181270.13392556997974897No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG166860.12327920012589462No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC165370.12217836105009705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT44600.063.371246
AGACCGC47950.059.26014
CCGCGTT48400.058.6003467
CGCGTTC49150.057.6054348
GACCGCG62850.045.13245
GCGTTCT63550.044.8639839
CTCAGAC121550.027.1672231
AATACGG58900.026.6820632-33
TACGGAG59150.026.3600834-35
ATACGGA56950.025.85736332-33
TGGAGTC153350.025.050722
GGAGTCT157700.024.3909323
CAATACG60000.024.336730-31
GAGTCTT155850.024.235774
GCAACGC61600.023.10379880-81
CAACGCC65850.023.04106582-83
AGTCTTG169400.022.9985475
TCGCTAT67150.022.8536054
CTCGCTA67300.022.4348853
TATCGCG4800.022.17147422-23
Produced by FastQC (version 0.11.5)