FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13535130
Sequences flagged as poor quality0
Sequence length105
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC19450.030.0533771
TGGCGCG49000.028.10362
GGCGCGT50700.026.964273
TATACGC13500.024.578287
TGCGCGA35000.023.9100769
GTCTAAC38200.022.1751
TACGCTA14250.021.8921289
GGTGGCG50600.020.656791
GTGCGCG43100.019.5321488
ATATACG16550.019.4503486
GGGCGAT23050.018.9122261
TATCGCG6450.018.80738822-23
CGATCTG31600.018.339453
ATACGCT18000.018.1572388
CGGTAGC48550.018.1555378
CCGGTAG48100.018.1208277
GGCCGGT49100.018.055895
CCGGCGA24400.017.0471489
GCGTGCC85800.016.5655786
GCCGGTA53700.016.4156386
Produced by FastQC (version 0.11.5)