FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16635146
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAGAATCTCGTAT292700.1759527689146822TruSeq Adapter, Index 5 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA224650.022.2096549
AGAGCAC231150.021.4293778
GATCGGA303300.016.9351061
AAGAGCA330100.015.6589017
TCGGAAG343150.015.0075363
GAAGAGC356100.014.5358116
ATCGGAA397100.013.1733982
GGAAGAG440750.011.8094135
CGGAAGA496000.010.4369264
AGTCACA120200.08.25354325-29
CTCGTAT120600.08.20216140-44
TCTCGTA124600.07.84640340-44
GTCACAC128250.07.746711325-29
TCACACA128600.07.703206530-34
CAGTCAC139550.07.418647325-29
AATCTCG144550.06.78340740-44
CACACAG153050.06.754842330-34
CCAGTCA164800.06.51789425-29
TCGTATG156900.06.442267445-49
GAATCTC162350.06.208184240-44
Produced by FastQC (version 0.11.5)