FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16635146
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATTCGCACCGTGTAGATCTCGGT335390.2016153029255048Illumina Single End PCR Primer 1 (96% over 30bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT271750.018.4643258
GAGCGTC272350.018.2386179
AAGAGCG309800.016.3823647
GATCGGA307900.016.185661
TCGGAAG348650.014.3485893
GAAGAGC355400.014.26036556
ATCGGAA396050.013.0693592
GGAAGAG442550.011.74492555
CGGAAGA499250.010.4815424
TAGATCT98600.010.32372440-44
AGATCTC119150.08.77276440-44
GTAGATC129200.08.19147435-39
TCGCACC143450.07.306547630-34
GTGTAGA150700.07.19480435-39
TTCGCAC152100.06.948771525-29
TCTCGGT162000.06.75448940-44
AGATTCG160500.06.656869425-29
ATTCGCA162300.06.65401725-29
CCGTGTA165700.06.465285335-39
TGGTCGC168250.06.45981350-54
Produced by FastQC (version 0.11.5)