FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15872301
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACC16050.014.8017767
GTTAGAC17050.014.7780996
CAACACT64400.011.7375758
TAGTTAG22100.011.0827514
AACACTA71750.010.5351889
TAGACCC29200.09.1220788
TAGACTC21250.07.182165135-139
AGTTAGA36250.07.14948655
GCAACAC106300.07.11100447
ACACTAG20400.07.05799970-74
GACTCTA26750.05.759283135-139
GTTTCAG142050.05.70492941
CACTAGC29950.05.336271370-74
AGCAACA161550.05.25835566
Produced by FastQC (version 0.11.5)