FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005068944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005068944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16105664
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA67000.027.9373369
GCCACTC74650.024.592178
AAGCCAC116900.016.3199146
AGCCACT118200.016.0795147
ACAAAGC170850.011.503683
GGCCTAA34600.011.23570675-79
TACAAAG196650.010.2510752
AAAGCCA231050.08.724465
CTCACTA45400.08.53143515-19
ATACAAA266600.08.2268361
ACTCACT55000.07.40882615-19
CAAAGCC279500.07.392514
TACACAC59500.06.993383450-54
ACACACT61850.06.68111150-54
TAGCCAC64050.06.587661725-29
GGGCCCC62350.06.582145110-114
CCCTTAA74600.06.56238844
CTACACA60900.06.548908750-54
TAGGGGC62800.06.397179105-109
GCCCCTA63650.06.2893457110-114
Produced by FastQC (version 0.11.5)