FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15614688
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA78550.015.3074477
ACACTAT79200.015.08
CACTATA81050.014.9241229
CAAACAC107050.011.8374595
AAACACT125000.010.19526
CCAAACA136100.09.7340194
ACCAAAC149150.08.834063
TAGCCCT67700.06.9128512
GGCCCCT41700.06.215827585-89
GCCCTTA74450.05.8025524
TGGCCCC48050.05.45431885-89
GGCCCCA49950.05.4198245-49
AGGCCCC50300.05.41073545-49
CCCCTCA53200.05.30526385-89
GTAGCCC86450.05.08039331
Produced by FastQC (version 0.11.5)