FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15050325
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTCT65450.010.7029771
CACTCTT87750.08.5249842
GATCGGA277300.06.8943671
AGAGCGT273950.06.58975658
TCGGAAG318300.06.34850743
GAGCGTC294350.06.23002349
AAGAGCG310350.06.18753677
ATCGGAA352900.05.7070772
GAAGAGC357400.05.5743426
TCCCTAC52900.05.43773279
ACTCTTT145700.05.1330533
GGAAGAG392350.05.13226945
Produced by FastQC (version 0.11.5)