FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13951323
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATAG39500.010.5708149
AACACTA43000.010.212666
ACACTAT43750.010.0375867
CCTAGAC9450.07.9246407120-124
CACTATA56850.07.59798248
CCACTCA38400.07.49905069
ACCACTA55800.07.3539078
CACCACT62600.06.7850837
CAAACAC81450.05.9246414
AAACACT79250.05.81377465
CCACTAC79250.05.5412549
GCCACTC54400.05.4257838
ACAACAC81950.05.2743743
ACACCAC88100.05.06632766
ATACAAC89150.05.0476681
Produced by FastQC (version 0.11.5)