FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069364

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069364
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186147
Sequences flagged as poor quality0
Sequence length5-46141
%GC63

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAACCU350.0037598768179.13364480-489
UACCCCG550.0175.222291
UUAGAUU7700.0175.222271
AGCCCCG2000.0144.558381
CGUACGG150.0074217436142.773711
UUGUAGA2450.0139.859971
UAGAUUA405.840775E-8134.322
UGUAGAU2700.0131.335112
CAAGAUU7700.0130.721371
CGUAGAU1900.0129.62351
CUAGAUU1500.0128.496341
UAGAUUG13200.0125.365332
GUCCAUA354.8583097E-6122.806872
CGUCCUA354.9437185E-6122.377481
UGCAGAU2950.0119.784731
GUAGAUU3900.0104.870553
ACAGAUU3500.0104.020841
UUGUGUG1800.0102.370396
GAUUGUG26600.0101.485534
AUUGUGU25050.099.386485
Produced by FastQC (version 0.11.5)