FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069385

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069385
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7867277
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACC14600.020.246021
TAGCGCG807.406035E-519.743731
TTAGTCA22700.019.62543545
TCCGTCG1153.3611632E-617.658991
CGACCCG27100.016.60443523
GCGACCT20500.016.2446352
CGTGGCG29300.015.58745918
ACGCGCA1754.7075446E-815.4726381
ATTAGTC30450.015.29489444
GGCGACC29500.015.17691321
TGTCGCG1055.9130526E-415.0428441
TCAGACG30100.015.02358813
GCGACCC30200.014.89962922
CCGCTGA30350.014.82627327
ACCCGCT31500.014.42784625
TGGCGAC31200.014.42189220
CAGACGT32850.014.3822914
GTGGCGA32150.013.99574219
ACGTGGC32850.013.83448117
ACGCGAC3450.013.734771
Produced by FastQC (version 0.11.5)