FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5598871
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA214240.38264857325700125No Hit
CGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAAGTCTTTGGG104550.18673407549486318No Hit
GGGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTG97340.17385647927948333No Hit
ATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTC87570.15640653267417665No Hit
GGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACG87230.15579926738801447No Hit
CAGCCCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAA85020.15185204302796046No Hit
CCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACC79580.14213579844936597No Hit
CACCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAG75510.13486647575913072No Hit
GGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGAC74020.13220522494624362No Hit
CCCCCTCCCCAATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTT70370.12568605349185577No Hit
CAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCAT66060.1179880729525649No Hit
GGCAGCTTCCGGGAAACCAAAGTCTTTGGGTTCCGGGGGGAGTATGGTTGC60010.10718232300762065No Hit
ACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCA56250.10046668337241561No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCA55250.030.13615445
TAACGCG602.3635948E-730.083431
CGTCGAC1450.026.4526711
TAGTCAG17700.025.93255445
CGCGACC45450.022.6370331
ATTAGTC75850.021.95053544
TCGCGTA600.006409980418.8021431
TTACGCG851.2056213E-418.5291652
TCGCGAC5750.018.442451
GCGACCT68900.018.4177512
CGTGGCG97400.017.37019218
ATATTAG95950.017.32879842
CGCTGAA97100.017.21594228
CAGACGT98500.017.17620814
TCAGACG98850.017.13815313
CCGCTGA98050.017.11797727
GTGGCGA98800.017.07851219
CATATTA98050.017.0723941
CGACCCG99100.017.0274223
GCGACCC99150.017.01852822
Produced by FastQC (version 0.11.5)