FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11242897
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5300.014.02736545
TCTCGTA4600.013.71275442
CTCGTAT4750.013.2796643
TCGTATG5400.012.93273744
TATCTCG6150.010.62305240
ATCTCGT7450.09.37414941
ATCGGAA9001.0913936E-117.00761651
ACACGTC9953.6379788E-126.77952212
CGTCTGA10301.8189894E-126.767726415
TCGGAAG10701.2551027E-106.10449462
CGGAAGA12602.401066E-105.53719853
ACGTCTG12151.7922503E-85.18203114
ATCGCGC5750.0088292015.089906731
Produced by FastQC (version 0.11.5)