FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5860848
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1601.593071E-816.89559645
TCTCGTA2851.027729E-811.8559540
CTCGTAT2553.714522E-711.48399941
TCGTATG2851.5576861E-610.27515742
CGTATGC2901.9468225E-610.09791343
ACACGTC3401.5037822E-69.25907812
CGTCTGA4102.1088617E-68.22712915
ATCTCGT3909.46826E-68.07863339
ATCGGAA4101.8249493E-57.69105531
TCGGAAG4152.1443379E-57.59832672
TCACGAT3754.273293E-47.20564734
CATCACG5909.993291E-76.869790632
CGGAAGA5451.5936426E-56.60808853
ACGTCTG5401.0077319E-46.246523414
CACGTCT6352.3203196E-56.020271313
GCACACG6159.426119E-55.85010310
GGAGTAT9555.2040923E-75.4245811
CACGATC6753.5047645E-45.33751635
ACGATCT6753.5047645E-45.33751636
ATCACGA7809.080601E-55.196379733
Produced by FastQC (version 0.11.5)