FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23177834
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7850.015.78520645
CGTATGC9850.012.80842943
CTCGTAT9550.012.73908441
TCTCGTA10850.011.42038740
TCGTATG11350.010.32175342
ATCTCGT15700.08.31279639
ACACGTC18950.06.526069612
ATCGGAA19100.06.48580071
CGTCTGA19250.06.19097815
TCGGAAG23750.05.68985562
GTATGCC25250.05.621125744
CGGAAGA27400.05.092273
ACGTCTG26250.05.054018514
Produced by FastQC (version 0.11.5)