FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13610645
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4300.015.71591845
CTCGTAT5000.013.96580141
TCTCGTA5300.011.47524840
CGTATGC6350.010.64184443
TCGTATG6900.010.12010942
ATCTCGT7700.09.05946639
ACACGTC9700.08.3455712
TCGGAAG11700.06.92946532
CGTCTGA10450.06.88617615
ATCGGAA9701.0913936E-116.7332591
CATCTCG10753.6379788E-126.48741438
ACGTCTG12351.4370016E-105.644677614
CGGAAGA14155.456968E-125.56649263
CACGTCT14852.3646862E-115.300123713
GTATGCC15805.456968E-125.27489744
Produced by FastQC (version 0.11.5)