FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7606633
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG3000.025.52595545
CTCGTAT3700.020.0878641
CGTATGC4300.017.80845343
TCTCGTA3850.017.55019840
TCGTATG4900.016.08757442
ACACGTC6050.013.75296812
TCGGAAG5550.013.3892622
ATCGGAA5750.012.9239731
ATCTCGT5050.012.92194839
CGTCTGA6200.012.33235115
ACGTCTG7600.011.83600314
CACGTCT8700.09.82252213
CGGAAGA7850.09.4600053
AATCTCG9400.07.89782538
GTATGCC10100.07.804811544
CACACGT11400.07.495986511
GCACACG10650.07.390409510
GTCACAC16400.05.48530128
Produced by FastQC (version 0.11.5)