FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069501

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069501
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9158355
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4450.012.15188445
CTCGTAT4551.8189894E-1210.89405243
TCGTATG5301.8189894E-129.77787345
TCTCGTA4752.728484E-109.48684242
TATCTCG5007.2759576E-119.46312441
TCCGTAT5751.4551915E-118.99856837
CCGTATC5151.1972588E-88.29883438
GATCGGA5709.677024E-108.2979451
AGTTCCG5751.1496013E-98.22556433
TTCCGTA6906.566552E-107.507459635
ATCTCGT6551.42918E-87.22375941
CGTATCT6903.9693987E-86.84871939
ACACGTC8002.0972948E-96.745425712
CGTCTGA8202.462366E-86.306905316
GTTCCGT8707.974813E-85.954191734
ATCGGAA8301.2588862E-65.6985891
AAGCGGC6451.8278098E-45.586946533
TCGGAAG9301.7968741E-65.3278452
CGGAAGA11301.0071926E-75.1780623
AAAGCGG7005.846993E-45.14797232
Produced by FastQC (version 0.11.5)