FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13785221
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5350.012.20981243
TCGTATG5850.011.93632444
TCTCGTA6150.010.25532142
CGTATGC6800.09.93753445
GTCACGA6350.09.56154328
ACGAGTG8450.08.52518931
TATCTCG7850.08.31982540
ATCTCGT8450.07.729040641
CACGAGT10400.06.926263330
AGTCACG9151.8189894E-116.881369627
CGTCTGA11057.2759576E-126.308800715
ACACGTC10902.1100277E-105.982998412
ATCGGAA12352.1827873E-115.84289031
CGAGTGG15350.05.574539732
TCGGAAG14201.9099389E-105.2342832
Produced by FastQC (version 0.11.5)