FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12601437
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5250.021.02883345
CTCGTAT5800.018.64225641
TCTCGTA6300.017.52026740
CGTATGC6750.017.02327343
TCGTATG7450.014.81897742
ATCTCGT8600.013.34579839
CGTCTGA10450.011.18952415
ATCGGAA10300.011.1650841
ACACGTC10950.010.47322912
ACGTCTG12050.010.26361214
TCGGAAG12450.08.8647262
CACGTCT14250.08.52125313
CGGAAGA14100.08.3009823
GTATGCC16450.07.67006144
AATCTCG17650.07.00911538
GCACACG18200.06.6718610
CACACGT20300.06.313982511
Produced by FastQC (version 0.11.5)