FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069524

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069524
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7335129
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2502.8718932E-711.71439444
TCTCGTA2952.4209658E-69.92745143
TCGTATG3301.0071555E-58.8745445
CGTATCT4354.8551956E-67.75942239
CCGTATC4152.1831904E-57.58814338
TCCGTAT4202.0710376E-46.963057537
AGTTCCG4801.4570243E-46.568509634
GTTCCGG3800.00381660376.52718831
TTCCGTA4901.9048968E-46.434458336
ACTCGAT4250.00180171456.34891948
TATCTCG5350.00376703755.473268541
ATCTCGT5700.0079132935.137892242
Produced by FastQC (version 0.11.5)