FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11056075
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGC121260.10967725888256005TruSeq Adapter, Index 25 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC16650.034.26077345
CTCGTAT18550.030.63100843
TCGTATG18700.030.50560444
TCTCGTA17500.028.86121742
TATCTCG18750.027.37321740
ATCTCGT19800.026.26267841
CGTCTGA23150.025.54417815
ACACGTC23150.025.4469412
ATCGGAA25450.023.4401381
ACGTCTG26450.022.69712414
TCGGAAG26600.022.4875532
CACGTCT28050.021.72309313
CGGAAGA27950.021.6422713
GCACACG30400.019.60015510
CACACGT31450.018.87428311
GTCACAC39200.014.91352728
TCTGAAC46200.013.28671617
CAGTCAC44950.013.25584226
AGAGCAC45950.013.1140387
AGCACAC47700.013.0100179
Produced by FastQC (version 0.11.5)