FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12854887
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4950.018.67478245
TCGTATG7000.013.85008142
CGTATGC6700.013.797243
CTCGTAT6750.013.67366541
TCTCGTA7000.011.89890140
ATCTCGT9000.010.00469239
ATCGGAA10900.09.0876451
TCGGAAG11700.09.033452
ACACGTC11550.08.95453812
TCACGAT9650.08.8736634
CGTCTGA11900.08.31335715
CATCACG14750.07.791564532
ACGTCTG14300.06.918054614
CGGAAGA15800.06.54684833
CGAATCC6302.951847E-66.43216371
GTATGCC15850.06.401230344
ACGATCT16700.06.207087536
CCCGGAT4400.00262519166.1397931
CACGATC17650.06.128342635
CACGTCT16800.06.022413713
Produced by FastQC (version 0.11.5)