FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12360902
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4600.017.15557145
GATCGGA4700.012.9318931
CGTATGC6550.012.7370743
CTCGTAT6200.012.70863541
TCTCGTA6800.011.25631140
TCGTATG7150.011.03750942
ACACGTC9200.09.53123412
CGTCTGA9150.09.09190315
ATCGGAA8650.08.5880391
ATCTCGT9350.08.1860139
TCGGAAG10350.07.6047092
CGGAAGA10600.07.2130253
CATCTCG11150.07.059080638
ACGTCTG12850.06.648946314
CACGTCT14150.06.19698613
CGGAGTC19700.05.93491845
GTATGCC15750.05.72642144
AGACGGA23750.05.68123252
ACGGAGT24550.05.6782484
GAGACGG52250.05.29925731
Produced by FastQC (version 0.11.5)