FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15214863
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG193000.12684964695377146TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28600.038.8631645
CTCGTAT30700.036.075141
CGCCAAT31300.035.7024832
CGTATGC31250.035.5682643
TCGTATG31950.034.64785842
TCTCGTA29150.034.20944240
GTCACGC32800.033.79668428
TATCTCG30150.033.64021338
ACGCCAA34300.032.44566731
AGTCACG34650.032.1869227
CGTCTGA35750.031.38493315
ATCTCGT32600.031.13946739
ACACGTC37000.030.14222312
ATCGGAA37450.029.9959351
TCGGAAG40300.027.9007532
ACGTCTG40600.027.58027614
CACGTCT41750.026.8205813
CGGAAGA42100.026.7074093
GTATGCC42450.026.343144
CACACGT47350.023.60114711
Produced by FastQC (version 0.11.5)