FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17988448
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC8950.010.32095544
TATGCCG5750.010.18774145
CTCGTAT8750.010.042006542
TCTCGTA9650.010.03939341
GTCACGA10450.09.25299529
TCGTATG11500.08.22830843
GATCGGA12050.08.0373841
AGTCACG12500.07.915398628
CGTCTGA13650.07.41296416
CGATCAG12950.07.303920333
ACACGTC13100.07.20926913
ATCTCGT14050.07.05574240
TCACGAT13000.06.926756430
CTACTCG54800.06.821878434
GTAGTCC88800.06.735761624
ATCGGAA14450.06.7021942
TACTCGG54900.06.686390435
TAGTCCC95500.06.475113425
ACGATCA15000.06.45585432
GCTACTC128450.06.36427633
Produced by FastQC (version 0.11.5)