FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15538710
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4701.8189894E-1210.5542545
CTCGTAT6951.8189894E-128.42152941
CGTATGC6602.910383E-118.19935543
TCTCGTA7101.209628E-97.2923340
TCGTATG7555.620677E-107.16764842
GTAGTCC76950.06.340602424
TACTCGG47600.06.24853435
GGGCGCC29500.06.17359716
CTACTCG48900.06.128496634
TATCTCG8851.819717E-86.0983838
TAGTCCC82150.06.02135925
GAGACGG96200.05.9678891
GGTGGCG50950.05.957433710
GGCGCCT36450.05.860106517
TGTAGTC91750.05.75892223
TGGCGGG36350.05.75238812
CTCGGGA50500.05.745955537
GTGGCGG36400.05.682718311
TCGGGAG55650.05.616893338
AGCTACT181300.05.56789932
Produced by FastQC (version 0.11.5)