FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14250864
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13150.028.46149445
CTCGTAT13700.027.9776643
TCTCGTA12400.026.00143842
TCGTATG15100.025.38370344
ATCGGAA17550.021.8090341
CGTCTGA18000.021.48536315
ATCTCGT15750.021.2958841
TCGGAAG18550.020.487932
ACACGTC19650.019.33797312
CGGAAGA21700.017.8245243
ACGTCTG21950.017.82384714
CACGTCT23350.016.466313
GCACACG28450.013.75156810
AATCTCG24800.013.61527440
CACACGT30800.012.62942711
AGTCAAC34100.011.8269633
GATCGGA7500.010.8070181
GTCAACA41200.09.51538834
AGAGCAC45800.09.1313027
ACTCGTA4853.92356E-98.83272842
Produced by FastQC (version 0.11.5)