FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11121809
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG158180.14222506428585494TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA20200.039.9006231
CTCGTAT24400.031.59827244
TCTCGTA23050.030.9061743
CGTCCCG25850.030.42163335
ATCGGAA26250.030.412272
TCGTATG25500.030.41147845
CCCGATC24150.029.42535438
GTCCCGA26700.029.0311936
CCGATCT24750.028.83074239
TCGGAAG27700.028.5762253
TCCCGAT25350.028.57330737
ACACGTC28100.028.24553713
ATCTCGT25550.028.05855442
CGTATGC8150.027.66060445
CGTCTGA28800.027.55913516
ACCCGTC28900.027.36698532
CACCCGT29300.026.91452831
CGGAAGA29800.026.4082164
TCACCCG29700.026.39187630
ACGTCTG30700.026.07312415
Produced by FastQC (version 0.11.5)