FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14163740
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10400.023.41399445
ACGTCCG10850.022.42821931
CGTCCGC11900.020.82956932
CTCGTAT12400.020.18352943
TCTCGTA11750.019.3811442
TCGTATG13300.018.47847444
GTCCGCA13400.018.32973733
ATCTCGT13150.017.63239941
TCCGCAC14300.016.86089934
CGCACAT15050.015.42175236
CCGCACA16100.015.11579135
GTCACGT16750.015.034348528
AGTCACG17300.014.55637727
ACACGTC18050.014.20047712
CGTCTGA18750.013.55041115
TCACGTC19900.012.88051429
CACGTCC19650.012.83779130
CATCTCG19000.011.96647340
ATCGGAA22150.011.6869451
ACGTCTG23750.010.69769314
Produced by FastQC (version 0.11.5)