FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14815479
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC14650.029.09254845
CTCGTAT15100.027.62902643
TCGTATG15700.026.86032944
GTCACGA16950.025.86628
TCTCGTA14600.025.64047442
ACGAGTG18650.023.68026431
TATCTCG16700.022.7813940
CACGAGT19500.022.64084830
ATCGGAA19350.022.4534651
CGTCTGA19850.021.86055415
AGTCACG19900.021.46668227
ACACGTC20250.021.31763812
TCGGAAG20750.020.8069112
ATCTCGT18850.020.30247541
CGGAAGA24100.018.1942733
ACGTCTG25700.017.1469114
CGAGTGG26200.016.94357732
CACGTCT26750.016.55790313
CACACGT31600.014.01662611
GCACACG31300.014.00716510
Produced by FastQC (version 0.11.5)