FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32102991
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC15500.012.93926745
GATCGGA14450.012.1652821
CTCGTAT16950.012.09826443
TCTCGTA19200.010.5632342
TCGTATG20400.010.4940944
TATCTCG20750.09.77421540
ATCGGAA21900.09.3646751
ATCTCGT24050.08.71415541
ACACGTC24000.08.71298312
CGTCTGA24500.08.07628715
TCGGAAG26550.08.0576722
CGGAAGA31100.07.52729943
ACGTCTG29700.07.116503214
CACGTCT35700.06.4243213
CACACGT44750.05.32608411
GCACACG40550.05.323234610
GAGACGG124850.05.21680641
CGAAGAT30450.05.10689831
Produced by FastQC (version 0.11.5)