FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21470308
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA19100.027.0187171
CTCGTAT22400.022.03213942
TCTCGTA22050.021.25770441
TATGCCG10350.021.11965845
CGTATGC24100.020.3842644
ATCGGAA25500.020.0464382
TCGTATG24750.019.84891743
TCGGAAG27050.018.7236963
ACACGTC27550.018.36316313
CGTCTGA27100.018.33620816
TCACGAT27500.017.27653935
ATCTCGT28900.016.5310240
CGGAAGA31850.016.3862114
ACGTCTG32300.015.31463815
CATCACG35500.014.27122133
CACGTCT36300.013.93678114
GCACACG40650.012.72198411
GTATGCC42300.012.25301645
CACACGT42250.012.02730412
ACGATCT41500.011.70676337
Produced by FastQC (version 0.11.5)