FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24488712
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11500.025.27353945
CTCGTAT13650.020.9629541
TCGTATG15000.019.3765842
CGTATGC15600.018.63113643
TCTCGTA14450.018.2431340
ATCGGAA18900.015.2630231
CGTCTGA22800.013.01802715
ACACGTC23750.012.87601312
ATCTCGT21050.012.72115639
TCGGAAG24200.012.4689552
CGGAAGA28250.010.6771563
ACGTCTG29450.010.07847414
CATCTCG29700.09.76892338
GTATGCC32000.09.71635344
CACGTCT35250.08.80290713
GCACACG45300.07.247071710
CACACGT47200.06.860067411
CTTAGGC46750.06.64661932
ACTTAGG54600.06.06070631
TTAGGCA56350.05.75402733
Produced by FastQC (version 0.11.5)