FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005069559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005069559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25767203
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG4203.1444688E-78.5849181
CTACTCG65100.07.263695234
TACTCGG64450.07.232138635
CTCGGGA67300.07.185609337
GTAGTCC108950.06.893860324
TCGGGAG73100.06.861050638
TAGTCCC116250.06.789925
GAGACGG117950.06.7061711
GCTACTC164600.06.66221233
GGTGGCG70350.06.584116510
CAGCTAC251100.06.544677731
AGCTACT257000.06.53619532
ACGGGGT74800.06.26192762
GCGTGGT82900.06.21119746
TGTAGTC133600.06.21062623
GACGGGG83100.06.2101191
CGGGCGT27900.06.13141633
CCAGCTA309300.06.09175630
GACGGAG63900.05.8895361
GGCGCAT18750.05.876113
Produced by FastQC (version 0.11.5)