FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5845524
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59060.1010345693559722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG6800.045.6655271
TGACCCG7050.040.620371
TAACCGG3550.038.876441
GACCGGA3200.034.502252
GACCGGT2950.032.74792
TGACGGG7100.032.5590171
GACCGGG7050.032.299982
GACCCGG6600.031.8884432
TGACGCG1700.030.4436841
TAACGCG802.762421E-630.1899871
GACCCGA4550.029.5733552
TTACCCG3650.028.3584981
TGACCAT37000.028.0685271
TTACCGG4200.027.9308721
TTACCGC3600.027.7939571
TGGCGGT6300.027.3832051
TGACCGC5550.027.3536031
TAACCCG4200.027.1093771
TGACCAG35000.026.9122141
GACCGGC4800.026.5954842
Produced by FastQC (version 0.11.5)