FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5845524
Sequences flagged as poor quality0
Sequence length75
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG3304.1354724E-813.5909169
GTCGCCG3355.0351446E-813.388288568
CTACTCG14650.011.06863937
TAGCCGG16950.010.9921852
TCGCCGG2550.001531571610.82352969
TTAGCCG17250.010.6019951
TCGTGTT13000.010.3569
TACTCGG15100.09.82475538
GTAGTCC36750.09.76276427
GCGTCGG9900.09.75757669
GGCGCCT12950.09.59089820
TCGTATA6252.0407242E-89.38327711
TCTCGGT9350.09.22475668
GCTACTC43200.09.02461436
CTTCGTA6503.8595317E-89.02261459
GTAGGCG14550.09.01030969
GGTCGCC5059.973242E-68.88134167
ATCTTCG8202.8194336E-108.8349887
TGGTCGC5503.0806095E-68.78189366
TAGTCCC39900.08.73263528
Produced by FastQC (version 0.11.5)