FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5107373
Sequences flagged as poor quality0
Sequence length75
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT2353.221725E-614.68131869
GCGTCGG6950.011.91404769
ATATCGG5005.16593E-1011.73037369
GTAGGCG11250.010.12032169
TATCGGT2750.002868462410.03668267
TGGTCGC3552.1938208E-49.71861869
TTCTTCG4301.4538864E-59.62736518
GTAGTCC28700.09.01543627
CAGCTAC52550.08.79744334
TCGTGTT10200.08.79439769
AGAGCGG17300.08.77484569
GCTACTC32750.08.74370436
TTAGCCG13950.08.657291
GGTCGCC3200.00998657858.62535968
GTGGTCG5201.4427176E-58.62527569
GAAAGAA160700.08.6102691
GGCGCCT10551.8189894E-128.5021420
AGCTACT54700.08.26252235
TAGTCCC31350.08.25336628
CCAGCTA58550.07.95468133
Produced by FastQC (version 0.11.5)