FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6187770
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67150.10852051708450702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG551.4703983E-743.912061
GACGGCG2500.042.7808152
TGACCGG6550.037.9262281
TTGCGCG1100.037.638911
TGACGGG9700.035.9251061
TGACGGC5850.035.3870051
GACCGCG2400.034.500662
TGACGCG2350.033.7682381
TGGCGGT6250.030.3620511
GACCGGG6150.029.7322752
TAACCGC3250.029.7250841
TGACCCG6050.029.084611
TGGCGGG12600.028.4781151
TAGCGCG854.4299395E-628.4136871
TACGCGG500.007736776527.6005272
GACCGGT2350.026.4260352
GACGCGG3200.025.8754942
TAGCGGC2700.025.5572811
TGCGCGA1359.436917E-925.5572811
TGGCGGC8400.025.0552651
Produced by FastQC (version 0.11.5)