FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5830076
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58550.10042750729150016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGG8550.038.3357241
TGACGCG1900.038.133961
GACCGCG1400.036.9662742
TGACCGG5650.036.6394541
TGACCGC4700.032.299891
TTACGCG652.3221422E-531.848141
ACGCGCG901.9969048E-730.6677913
TGACGGC5350.029.6654031
GACCGGG6050.029.6544882
TAGCGCG1052.3330358E-829.5732751
TGACCCG6450.029.4204391
TAACCGC2350.029.3635331
TAACGCG854.4300614E-628.4135381
GACGGCG2350.027.8951172
TTACCGC3600.026.8350071
GACGGGC4300.026.4781682
TGACGGT4750.026.1491
GACCCGG6400.025.8763922
TAGCCCG2900.024.9843181
TTCGCCG700.001334012824.6443941
Produced by FastQC (version 0.11.5)