FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6156248
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67510.10966094933147592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG6500.037.686161
TTACGCG754.0241503E-836.8015061
TAACCGC2700.034.5014151
TGACGGG8800.032.9331671
TGACCCG6500.032.9090421
TGCGCGA958.805728E-932.6855471
TTGCGCG751.6702634E-632.201321
GACCGGT2500.031.7410412
TGACGCG1650.031.364921
TTACCCG3450.031.0012721
TAGCGCG802.763345E-630.1887381
GACCGGG5900.028.6534842
GACCGCG2050.028.6106952
GGCGAAC2900.028.5526622
TAACCGG2350.027.8947621
GCGTTGG4650.026.7105563
GACCGGC3950.026.2033922
GACCCGC5050.025.2780572
GACGGGC4750.024.6955492
TGACCAT34750.024.4240231
Produced by FastQC (version 0.11.5)