FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6616139
Sequences flagged as poor quality0
Sequence length75
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT3309.094947E-1216.72697369
TCGTGTT21500.014.28113869
GTCGCCG3657.1304385E-1014.17782768
CGCCGTA2001.9840029E-413.79975269
TCGCCGG2557.509094E-613.52916869
CTATCGC2901.9617582E-613.08626962
TCGCCGT2406.377003E-512.93726768
TCGGAAC3408.7323315E-712.17625169
GCGTCGG13800.011.99978469
GTAGTCC43900.011.55227127
TAGATCG5451.7826096E-1011.39429169
GCGATAT4451.7060302E-710.85373869
GTCGCCC4804.1782187E-810.78105569
TAGTCCC49050.010.76135628
GATCGGG5805.293259E-1010.70670569
TACTCGG18800.010.64366738
TTAGCCG21100.010.6300211
TAGCCGG22100.010.6162372
CTACTCG18550.010.60128837
TGTAGTC54050.010.08515526
Produced by FastQC (version 0.11.5)