FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7273023
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76160.10471574199614109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG6150.039.8326261
TGACCCG7750.036.06111
TGACGCG2700.034.502911
TTACGCG807.0978786E-834.5029071
GACCGGG7600.034.047052
TGACCGT6050.033.0771681
TAGCGCG1154.7293724E-1133.002781
TAACGCG553.2571112E-431.366281
TGACGGG8800.031.366281
TAACCGG3700.030.7728631
TACGCGG901.9971594E-730.6675682
ACCGGGG7050.030.3413143
TAACCCG3650.030.2491251
TGGCGGG13400.028.8382531
TGACGGC6150.028.0511461
TAACCGC3700.027.975331
TGACCAT45200.027.3275241
GGACCGG5950.027.2543981
GACCGCG2800.027.1079372
GACCCAA32500.026.8577082
Produced by FastQC (version 0.11.5)