FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9990164
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105660.10576402949941562No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG12000.039.6772161
TGACCCG11400.037.5284161
GACCGGG9550.036.4873052
TGACGCG3350.036.046791
TGACGGG12000.030.7642171
TGACGGC8500.028.8192581
GACCGGT5300.027.9908662
GACCCGT6150.027.48812
GACCCGG11000.027.286662
GACCGCG4700.027.1598682
TGACCAT60850.026.9324821
GACCGGC7450.026.8593522
TGACCAG58450.026.7397291
GACCCAA43250.026.6430632
TGGCGGG19100.026.553841
TGACCCA68000.026.282351
TGACCGC10400.026.2081951
GGACCGG8250.025.928721
GACGCGG4000.025.8752782
TGACCTG65700.025.4169431
Produced by FastQC (version 0.11.5)