FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8314507
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92190.11087849225456181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG8700.040.4500471
AATCGCG1550.035.61403338
TGACCCG9700.035.212881
TAACGCG1253.6379788E-1233.121451
TGACGCG2400.033.0639461
GACCGGG8200.031.1345732
TTACCCG5450.030.386651
TGACGGG8950.027.7554051
TGACCAT54050.027.703341
TAACCGC3800.027.2380331
TAACCCG5500.026.3466051
GACCCGG9200.025.8753532
GACCGGA5250.025.6289232
TAACCGG4050.025.5566751
GACCCAA37100.025.387142
GACCCGA7100.025.2679522
TGACCAG46900.025.0853181
GACCGGT4300.024.8724352
TAGCCCG3750.024.8410871
GGACCGG6500.024.4164521
Produced by FastQC (version 0.11.5)