FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5312741
Sequences flagged as poor quality0
Sequence length75
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA1450.00521771314.27605368
CTACTCG12700.012.76740637
TAGATCG3752.1929918E-711.96015969
TACTCGG13050.011.89626238
TCGTGTT15500.011.35176569
GATCGGG5855.0931703E-1111.20527869
GTAGTCC30600.010.59768327
TCTCGGG6857.2759576E-1210.57678469
GCGCCTG16300.010.37101121
GCTACTC34750.010.22565136
TAGTCCC34600.010.17017728
GCGTCGG11600.010.11220569
TAGCCGG14800.010.0249152
GGCGCCT12050.09.73429520
TTAGCCG14300.09.6523871
TGTAGTC37400.09.5933526
AGCTACT55150.09.44582735
CAGCTAC54500.09.30527934
GAAAGAA180350.09.2414851
ACTCGGG14950.08.99978139
Produced by FastQC (version 0.11.5)