FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6415417
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67820.10571409465666846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCG758.731149E-1041.4009862
TGACCGG7350.038.4922141
TTACGCG1201.8189894E-1234.5021671
TGACGGG7500.034.0421371
TGACCCG7350.033.7980421
GACCGGT2800.033.268652
GACCCGG6700.032.956012
TGACGCG2250.032.2020231
GACCGGG7400.031.237232
GACGCGG2800.029.5721342
TTACCGG3650.029.3032131
TAACCGG3350.028.8376311
TAGCGCG756.16521E-527.6017341
TGACCGC6900.027.5017261
TGACCAT41900.026.9265121
GACCCAA27050.026.9119172
GGACCGG5400.026.8350181
TAACCGC2200.026.6607671
TGGCGGC7600.026.33061
TGGCGGG11550.025.9886441
Produced by FastQC (version 0.11.5)