FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8227393
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87130.10590231924012869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG9850.043.4334141
GACCGGG8800.040.3817562
TGACGGG9600.039.1736261
TGACGCG2450.038.022111
TAACGCG1051.6370905E-1136.1444741
TGACCCG8750.033.910091
TTGCGCG1450.033.3118321
TAACCGG4250.033.2838441
GGACCGG6050.031.9353141
TAGCCCG4800.031.6264131
TCGCGTA553.2579023E-431.3650421
GACCGGA4100.030.2934862
GACGCGG2400.030.18832
TGACCGC8500.030.0366381
GACGGCG2800.029.5722122
GACCCGA5650.028.6998772
GACCGGC6450.028.3495882
GACCGGT3800.028.1454832
GAATCGG7600.028.14462937
ATCGGGG7500.028.06040439
Produced by FastQC (version 0.11.5)