FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8227393
Sequences flagged as poor quality0
Sequence length75
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG5900.016.95755869
CGCCGTA2101.814758E-514.78565367
GGCGCCT16500.012.12692720
TAGCCGG22650.011.8819732
TTAGCCG20600.011.72553351
CTACTCG19500.011.50023237
TCGCCGT3003.9838764E-511.49995266
GCGTCGG15600.011.49995169
TCTCGGG7300.011.34241969
TACTCGG18900.011.3176938
GTAGTCC49600.011.26775627
CGGGCGT12000.010.637656
CAGCTAC91200.010.51672434
GTCGCCG4108.25461E-610.09751968
AGCTACT95950.09.99609335
GCTACTC57350.09.98631236
TAGTCCC55050.09.90156628
GTGGTCG11500.09.8999669
TCGTGTT22150.09.81260169
TGTAGTC62200.09.70643326
Produced by FastQC (version 0.11.5)