FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005071983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005071983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6509280
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67740.1040668092323575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACCGG6700.042.740251
TGACGCG2800.041.8942761
TTGCGCG856.730261E-1140.589611
TGACGGG8650.039.0880281
GACCGCG1800.036.4173362
TAGCGCG1400.034.5011671
GACCGGG5800.034.5006372
GACGCGG2300.034.5006372
ACGCGGG1600.034.5003743
TGACCGC6450.034.2337151
GACCGGT2150.033.69832
TGCGACG1253.6379788E-1233.121121
GACCGGC4400.032.932432
TAACGCG1056.4392225E-1032.8582531
TGACGGC5100.032.4716871
GACGCGC2050.031.9762022
TGACCCG6550.031.0773871
GACGGCG2250.030.6672332
TAACCGG3500.029.572431
GACCCGG5350.029.019232
Produced by FastQC (version 0.11.5)